- Featured Product
- KD/KO Validated
ERG Polyclonal antibody
ERG Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HEK-293 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human prostate cancer tissue, mouse brain tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14356-1-AP targets ERG in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB,ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | ERG fusion protein Ag5708 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | v-ets erythroblastosis virus E26 oncogene homolog (avian) |
Synonyms | ERG, erg 3, p55, Transcriptional regulator ERG, Transforming protein ERG |
Calculated Molecular Weight | 55 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC040168 |
Gene Symbol | ERG |
Gene ID (NCBI) | 2078 |
RRID | AB_2098423 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P11308 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ERG antibody 14356-1-AP | Download protocol |
IHC protocol for ERG antibody 14356-1-AP | Download protocol |
IF protocol for ERG antibody 14356-1-AP | Download protocol |
IP protocol for ERG antibody 14356-1-AP | Download protocol |
FC protocol for ERG antibody 14356-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Biosci Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer | ||
J Cancer miR-223-5p targeting ERG inhibits prostate cancer cell proliferation and migration.
| ||
Cell Death Discov HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells. | ||
Exp Ther Med Rare angiolymphoid hyperplasia with eosinophilia examined through fine needle aspiration cytology, histopathology and immunophenotypic characterization: A case report | ||
Nat Commun DNA framework-engineered chimeras platform enables selectively targeted protein degradation |