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ERG Polyclonal antibody

ERG Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14356-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

erg 3, Transcriptional regulator ERG, Transforming protein ERG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存


经过测试的应用

Positive WB detected inMCF-7 cells
Positive IP detected inMCF-7 cells, rat heart tissue
Positive IHC detected inhuman prostate cancer tissue, mouse brain tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14356-1-AP targets ERG in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 ERG fusion protein Ag5708 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-ets erythroblastosis virus E26 oncogene homolog (avian)
别名 erg 3, Transcriptional regulator ERG, Transforming protein ERG
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC040168
基因名称 ERG
Gene ID (NCBI) 2078
RRIDAB_2098423
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11308
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERG (ETS-related gene) is a member of the ETS family of transcription factors, which are downstream effectors of mitogenic signaling transduction pathway. ERG is a key regulator of cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. ERG is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia.

实验方案

Product Specific Protocols
WB protocol for ERG antibody 14356-1-APDownload protocol
IHC protocol for ERG antibody 14356-1-APDownload protocol
IP protocol for ERG antibody 14356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Stem Cell

PSPC1 exerts an oncogenic role in AML by regulating a leukemic transcription program in cooperation with PU.1

Authors - Juyeong Hong
mouseIF

iScience

Resident vascular Sca1+ progenitors differentiate into endothelial cells in vascular remodeling via miR-145-5p/ERG signaling pathway

Authors - Zhangquan Ying
humanWB

Cell Biosci

Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer

Authors - Qiong Wang
humanWB

J Cancer

miR-223-5p targeting ERG inhibits prostate cancer cell proliferation and migration.

Authors - Yongbao Wei
  • KD Validated
mouse,humanWB,IF

Cell Death Discov

HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells.

Authors - Feng Yao
humanIHC

Exp Ther Med

Rare angiolymphoid hyperplasia with eosinophilia examined through fine needle aspiration cytology, histopathology and immunophenotypic characterization: A case report

Authors - Xiaohua Wang
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