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验证数据展示

  • WB analysis of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)

    WB analysis of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)

    WB result of ERCC2 antibody (10818-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERCC2 transfected HEK-293 cells. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 10818-1-AP (same clone as 10818-1-PBS)

    WB analysis of HeLa using 10818-1-AP (same clone as 10818-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)

    IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ERCC2 antibody included
  • IP experiment of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)

    IP experiment of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)

    IP result of anti-ERCC2 (IP:10818-1-AP, 4ug; Detection:10818-1-AP 1:1000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

ERCC2 Polyclonal antibody, PBS Only

ERCC2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10818-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

BTF2 p80, COFS2, CXPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ERCC2 Ready-To-Use IHC Kit. ERCC2 primary antibody included.

产品信息

10818-1-PBS targets ERCC2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1038

Product name: Recombinant human ERCC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC008346

Sequence: MRELKRTLDAKGHGVLEMPSGTGKTVSLLALIMAYQRAYPLEVTKLIYCSRTVPEIEKVIEELRKLLNFYEKQEGEKLPFLGLALSSRKNLCIHPEVTPLRFGKDVDGKCHSLTASYVRAQYQHDTSLPHCRFYEEFDAHGREVPLPAGIYNLDDLKALGRRQGWCPYFLARYSILHANVVVYSYHYLLDPKIADLVSKELARKAVVVFDEAHNIDNVCIDSMSVNLTRRTLDRCQGNLETLQKTVLRIKETDEQRLRDEYRRLVEGLREASAARETDAHLANPVLPDEVLQEAVPGSIRT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 excision repair cross-complementing rodent repair deficiency, complementation group 2
别名 BTF2 p80, COFS2, CXPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells
计算分子量 87 kDa
观测分子量 80 kDa
GenBank蛋白编号BC008346
基因名称 ERCC2
Gene ID (NCBI) 2068
RRIDAB_2231330
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18074
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Excision-repair complementing defective in Chinese hamster 2(ERCC2), belongs to the nucleotide excision repair pathway, is a component of the TFIIH transcription complex and required for the association of the CDK-activating kinase(CAK) subcomplex with TFIIH. Since TFIIH is involved in both DNA repair and cell cycle progression, ERCC2 is implicated to play an integral role in the nucleotide excision repair pathway. Also it involves in the regulation of vitamin-D receptor activity. As a part of the mitotic spindle-associated MMXD complex, ERCC2 has a role in chromosome segregation.

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