验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, COLO 320 cells, human testis tissue, Jurkat cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22211-1-AP targets ERC1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 | ERC1 fusion protein Ag17536 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ELKS/RAB6-interacting/CAST family member 1 |
| 别名 | ELKS, Cast2, ELKS/Rab6-interacting/CAST family member 1, ERC 1, ERC-1 |
| 计算分子量 | 1116 aa, 128 kDa |
| 观测分子量 | 124-135 kDa |
| GenBank蛋白编号 | BC132782 |
| 基因名称 | ERC1 |
| Gene ID (NCBI) | 23085 |
| RRID | AB_11232409 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IUD2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERC is an acronym based on previous separate namings of members of this protein family, ELKS, Rab6IP2 and CAST (PMID: 12391317). They are known to bind RIMs, the active zone proteins that regulate neurotransmitter release. ERC1 (ELKS) is an essential regulatory subunit of the IKK complex and likely functions by recruiting IκBα to the complex (PMID: 15218148). Multiple isoforms of ERC1 mRNA are generated by alternative splicing (PMID: 12203787). There are two known splice variants of ERC1 protein that differ at their C termini, ERC1a and ERC1b. ERC1a is ubiquitously expressed, whereas ERC1b is brain-specific (PMID: 12391317; 12923177).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERC1 antibody 22211-1-AP | Download protocol |
| IHC protocol for ERC1 antibody 22211-1-AP | Download protocol |
| IF protocol for ERC1 antibody 22211-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Facilitating identification of minimal protein binding domains by cross-linking mass spectrometry. | ||
J Cell Sci Organization and dynamics of the cortical complexes controlling insulin secretion in β-cells. | ||
J Biol Chem MICAL3 flavoprotein monooxygenase forms a complex with centralspindlin and regulates cytokinesis.
| ||
Nat Commun CLASP-mediated competitive binding in protein condensates directs microtubule growth | ||
