验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, mouse spleen tissue |
| Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11627-1-AP targets EPSTI1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | EPSTI1 fusion protein Ag2157 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | epithelial stromal interaction 1 (breast) |
| 别名 | Epithelial-stromal interaction protein 1 |
| 计算分子量 | 410 aa, 47 kDa |
| 观测分子量 | 35-38 kDa |
| GenBank蛋白编号 | BC023660 |
| 基因名称 | EPSTI1 |
| Gene ID (NCBI) | 94240 |
| RRID | AB_2877786 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96J88 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Real-time PCR revealed the highest expression in the placenta; strong expression in the small intestine, spleen, salivary gland, and testes; and low expression levels in normal breast and several other tissues. The expression of EPSTI1 was upregulated (range 5.6-72.1) in all 14 breast carcinomas tested. Microdissection revealed that both tumor and stromal tissue overexpressed EPSTI1. (PubMed: 11991720)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPSTI1 antibody 11627-1-AP | Download protocol |
| IHC protocol for EPSTI1 antibody 11627-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin EPSTI1 promotes monocyte adhesion to endothelial cells in vitro via upregulating VCAM-1 and ICAM-1 expression.
| ||
Elife KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition. | ||
Immunobiology Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations | ||
Mol Cancer Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study. | ||
Oxid Med Cell Longev Systematic Analysis of Molecular Subtypes Based on the Expression Profile of Immune-Related Genes in Pancreatic Cancer |
