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EPS8L2 Monoclonal antibody

EPS8L2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E3B1

Cat No : 68330-1-Ig

Print datasheet

Synonyms

EPS8 like 2, EPS8 like protein 2, EPS8 related protein 2, EPS8L2, EPS8R2, FLJ16738, FLJ21935, FLJ22171

验证数据展示

  • WB analysis of A431 using 68330-1-Ig

    WB analysis of A431 using 68330-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68330-1-Ig (EPS8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 68330-1-Ig

    WB analysis of HepG2 using 68330-1-Ig

    WB result of EPS8L2 antibody (68330-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS8L2 transfected HepG2 cells.

  • IF Staining of HeLa using 68330-1-Ig

    IF Staining of HeLa using 68330-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPS8L2 antibody (68330-1-Ig, Clone: 2E3B1 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of rat skin using 68330-1-Ig

    WB analysis of rat skin using 68330-1-Ig

    rat skin tissue were subjected to SDS PAGE followed by western blot with 68330-1-Ig (EPS8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat skin tissue, A431 cells, HepG2 cells, A549 cells，LNCaP cells，HeLa cells，HepG2 cells, mouse skin tissue
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68330-1-Ig targets EPS8L2 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen EPS8L2 fusion protein Ag16808 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name EPS8-like 2
Synonyms EPS8 like 2, EPS8 like protein 2, EPS8 related protein 2, EPS8L2, EPS8R2, FLJ16738, FLJ21935, FLJ22171
Calculated Molecular Weight 715 aa, 81 kDa
Observed Molecular Weight81-85 kDa
GenBank Accession NumberBC002474
Gene Symbol EPS8L2
Gene ID (NCBI) 64787
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H6S3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPS8L2 (epidermal growth factor receptor pathway substrate 8 L2), a member of the Eps8-like protein family, is a newly identified hair bundle protein that is localized at the tips of stereocilia of both cochlear and vestibular hair cells.

实验方案

Product Specific Protocols
WB protocol for EPS8L2 antibody 68330-1-IgDownload protocol
IF protocol for EPS8L2 antibody 68330-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols