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EPRS Polyclonal antibody

EPRS Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25307-1-AP

Print datasheet

Synonyms

EARS, EPRS, GLNS, GLUPRORS, GluRS Prolyl tRNA synthetase, Glutamate tRNA ligase, PARS, PIG32, Proline tRNA ligase, QARS, QPRS

验证数据展示

  • WB analysis of HEK-293 using 25307-1-AP

    WB analysis of HEK-293 using 25307-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25307-1-AP (EPRS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 25307-1-AP

    IF Staining of HEK-293 using 25307-1-AP

    Immunofluorescent analysis of HEK-293 cells using 25307-1-AP (EPRS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells
Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy EPRS Ready-To-Use IHC Kit. EPRS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25307-1-AP targets EPRS in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen EPRS fusion protein Ag18011 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamyl-prolyl-tRNA synthetase
Synonyms EARS, EPRS, GLNS, GLUPRORS, GluRS Prolyl tRNA synthetase, Glutamate tRNA ligase, PARS, PIG32, Proline tRNA ligase, QARS, QPRS
Calculated Molecular Weight 1512 aa, 171 kDa
Observed Molecular Weight 171 kDa
GenBank Accession NumberBC126275
Gene Symbol EPRS
Gene ID (NCBI) 2058
RRIDAB_2880023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07814
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)

实验方案

Product Specific Protocols
WB protocol for EPRS antibody 25307-1-APDownload protocol
IF protocol for EPRS antibody 25307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proteomics

Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.

Authors - Jing Zhang
View Article
humanWB

Nat Commun

Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA

Authors - Mirjana Malnar Črnigoj
View Article