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验证数据展示

  • WB analysis using 27252-1-AP (same clone as 27252-1-PBS)

    WB analysis using 27252-1-AP (same clone as 27252-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27252-1-AP (EPDR1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27252-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 27252-1-AP (same clone as 27252-1-PBS)

    IHC staining of human stomach using 27252-1-AP (same clone as 27252-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27252-1-AP (EPDR1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27252-1-PBS in a different storage buffer formulation.

EPDR1 Polyclonal antibody, PBS Only

EPDR1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27252-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

EPDR, Mammalian ependymin-related protein 1, MERP 1, MERP1, MERP-1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27252-1-PBS targets EPDR1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26120

Product name: Recombinant human EPDR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 52-160 aa of BC018299

Sequence: QVMYQQSSGRNSRALLSYDGLNQRVRVLDERKALIPCKRLFEYILLYKDGVMFQIDQATKQCSKMTLTQPWDPLDIPQNSTFEDQYSIGGPQEQITVQEWSDRKSARSY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Mammalian ependymin-related protein 1
别名 EPDR, Mammalian ependymin-related protein 1, MERP 1, MERP1, MERP-1
计算分子量 25 kDa
观测分子量25-30 kDa
GenBank蛋白编号BC018299
基因名称 EPDR1
Gene ID (NCBI) 54749
RRIDAB_3085942
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UM22
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ependymin-related 1 (EPDR1) gene, also known as MERP1 and UCC1, encodes for a protein related to ependymins, which are type II transmembrane proteins that are similar to two families of cell adhesion molecules: the protocadherins and ependymins. Ependymin-related protein 1 (EPDR1) was recently identified as a secreted human batokine regulating mitochondrial respiration linked to thermogenesis in brown fat. Proteomics studies of mammalian mannose-6-phosphate (M6P) glycoproteins have identified EPDR1 as a lysosomal protein with unknown function. The lysosomal localization of EPDR1 was further demonstrated in mouse brain homogenates by subcellular fractionation.(PMID: 36343918, PMID: 33902536, PMID: 30729188)

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