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验证数据展示

  • WB analysis using 32189-1-AP

    WB analysis using 32189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32189-1-AP (EPCAM/CD326 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 32189-1-AP

    IHC staining of mouse colon using 32189-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 32189-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 32189-1-AP

    IHC staining of mouse colon using 32189-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 32189-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse colon using 32189-1-AP

    IF Staining of mouse colon using 32189-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using EPCAM/CD326 antibody (32189-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat liver using 32189-1-AP

    IF Staining of rat liver using 32189-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using EPCAM/CD326 antibody (32189-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPCAM/CD326 Polyclonal antibody

EPCAM/CD326 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 32189-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Epithelial glycoprotein 314, Epithelial cell adhesion molecule, Ep-CAM, Epcam, EGP314

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse colon tissue, mouse small intestine tissue, rat colon tissue
Positive IHC detected inmouse colon tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat liver tissue, mouse colon tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32189-1-AP targets EPCAM/CD326 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epithelial cell adhesion molecule
别名 Epithelial glycoprotein 314, Epithelial cell adhesion molecule, Ep-CAM, Epcam, EGP314
计算分子量35 kDa
观测分子量40 kDa
GenBank蛋白编号NM_008532.2
基因名称 Epcam
Gene ID (NCBI) 17075
RRIDAB_3670218
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ99JW5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

实验方案

Product Specific Protocols
WB protocol for EPCAM/CD326 antibody 32189-1-APDownload protocol
IHC protocol for EPCAM/CD326 antibody 32189-1-APDownload protocol
IF protocol for EPCAM/CD326 antibody 32189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols