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EPB41L2 Polyclonal antibody

EPB41L2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15437-1-AP

Print datasheet

Synonyms

4.1 G, 4.1G, Band 4.1 like protein 2, EPB41L2

验证数据展示

  • WB analysis of HeLa using 15437-1-AP

    WB analysis of HeLa using 15437-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 15437-1-AP

    IP experiment of HEK-293 using 15437-1-AP

    IP result of anti-EPB41L2 (IP:15437-1-AP, 4ug; Detection:15437-1-AP 1:800) with HEK-293 cells lysate 2600ug.

  • IF Staining of HeLa using 15437-1-AP

    IF Staining of HeLa using 15437-1-AP

    Immunofluorescent analysis of HeLa cells using 15437-1-AP (EPB41L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary tumor using 15437-1-AP

    IHC staining of human ovary tumor using 15437-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPB41L2 antibody included
  • IHC staining of human ovary tumor using 15437-1-AP

    IHC staining of human ovary tumor using 15437-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPB41L2 antibody included

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EPB41L2 Ready-To-Use IHC Kit. EPB41L2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15437-1-AP targets EPB41L2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen EPB41L2 fusion protein Ag3647 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name erythrocyte membrane protein band 4.1-like 2
Synonyms 4.1 G, 4.1G, Band 4.1 like protein 2, EPB41L2
Calculated Molecular Weight 112 kDa
Observed Molecular Weight 130-150 kDa
GenBank Accession NumberBC034718
Gene Symbol EPB41L2
Gene ID (NCBI) 2037
RRIDAB_10951110
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43491
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EPB41L2 antibody 15437-1-APDownload protocol
IHC protocol for EPB41L2 antibody 15437-1-APDownload protocol
IF protocol for EPB41L2 antibody 15437-1-APDownload protocol
IP protocol for EPB41L2 antibody 15437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols