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验证数据展示

  • WB analysis of HeLa using 15437-1-AP

    WB analysis of HeLa using 15437-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 15437-1-AP

    IF Staining of A431 using 15437-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EPB41L2 antibody (15437-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IP experiment of HEK-293 using 15437-1-AP

    IP experiment of HEK-293 using 15437-1-AP

    IP result of anti-EPB41L2 (IP:15437-1-AP, 4ug; Detection:15437-1-AP 1:4000) with HEK-293 cells lysate 1610 ug.

  • IHC staining of human ovary tumor using 15437-1-AP

    IHC staining of human ovary tumor using 15437-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPB41L2 antibody included
  • IHC staining of human ovary tumor using 15437-1-AP

    IHC staining of human ovary tumor using 15437-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPB41L2 antibody included
  • IF Staining of HeLa using 15437-1-AP

    IF Staining of HeLa using 15437-1-AP

    Immunofluorescent analysis of HeLa cells using 15437-1-AP (EPB41L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

EPB41L2 Polyclonal antibody

EPB41L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15437-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Erythrocyte membrane protein band 4.1-like 2, EPB41 L2, Band 4.1-like protein 2, Band 4.1 like protein 2, 4.1G

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy EPB41L2 Ready-To-Use IHC Kit. EPB41L2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15437-1-AP targets EPB41L2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3647

Product name: Recombinant human EPB41L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 334-633 aa of BC034718

Sequence: ALLGSYTLQAELGDYDPEEHGSIDLSEFQFAPTQTKELEEKVAELHKTHRGLSPAQADSQFLENAKRLSMYGVDLHHAKDSEGVDIKLGVCANGLLIYKDRLRINRFAWPKILKISYKRSNFYIKVRPAELEQFESTIGFKLPNHRAAKRLWKVCVEHHTFYRLVSPEQPPKAKFLTLGSKFRYSGRTQAQTRQASTLIDRPAPHFERTSSKRVSRSLDGAPIGVMDQSLMKDFPGAAGEISAYGPGLVSIAVVQDGDGRREVRSPTKAPHLQLIEGKSSHETLNIVEEKSGQRLGKTKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 erythrocyte membrane protein band 4.1-like 2
别名 Erythrocyte membrane protein band 4.1-like 2, EPB41 L2, Band 4.1-like protein 2, Band 4.1 like protein 2, 4.1G
计算分子量 112 kDa
观测分子量 130-150 kDa
GenBank蛋白编号BC034718
基因名称 EPB41L2
Gene ID (NCBI) 2037
RRIDAB_10951110
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43491
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPB41L2, also known as 4.1G, is a member of the protein 4.1 family, which generally serves as adaptors linking transmembrane proteins to the cytoskeleton(PMID: 21482674).

实验方案

Product Specific Protocols
WB protocol for EPB41L2 antibody 15437-1-APDownload protocol
IHC protocol for EPB41L2 antibody 15437-1-APDownload protocol
IF protocol for EPB41L2 antibody 15437-1-APDownload protocol
IP protocol for EPB41L2 antibody 15437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols