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验证数据展示

  • FC experiment of THP-1 using 83078-4-RR (same clone as 83078-4-PBS)

    FC experiment of THP-1 using 83078-4-RR (same clone as 83078-4-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug p300 Recombinant antibody (83078-4-RR, Clone:230369H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83078-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83078-4-RR (same clone as 83078-4-PBS)

    IF Staining of HepG2 using 83078-4-RR (same clone as 83078-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-4-RR, Clone: 230369H8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83078-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00310-2

    Cytometric bead array standard curve of MP00310-2

    Cytometric bead array standard curve of MP00310-2, p300 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83078-4-PBS. Detection antibody: 83078-3-PBS. Standard: SY00911. Range: 0.313-5 ng/mL.

p300 Recombinant monoclonal antibody, PBS Only (Capture)

p300 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83078-4-PBS

产品说明书

CloneNo. 230369H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

EP300, 230369H8, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EP300 Ready-To-Use IHC Kit. EP300 primary antibody included.

产品信息

83078-4-PBS targets p300 as part of a matched antibody pair:

MP00310-2: 83078-4-PBS capture and 83078-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
亲和力
KD=1.30 x 10-8M
KOff=3.14 x 10-3M
KOn=2.42 x 105M
全称 E1A binding protein p300
别名 EP300, 230369H8, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48
计算分子量 264 kDa
GenBank蛋白编号NM_001429
基因名称 EP300
Gene ID (NCBI) 2033
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ09472
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).

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