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验证数据展示

  • WB analysis using 83945-5-RR (same clone as 83945-5-PBS)

    WB analysis using 83945-5-RR (same clone as 83945-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83945-5-RR (EOMES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83945-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83945-5-RR (same clone as 83945-5-PBS)

    IHC staining of mouse brain using 83945-5-RR (same clone as 83945-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83945-5-RR (EOMES/TBR2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83945-5-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 83945-5-RR (same clone as 83945-5-PBS)

    IF Staining of SH-SY5Y using 83945-5-RR (same clone as 83945-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using EOMES antibody (83945-5-RR, Clone: 241036D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83945-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83945-5-RR

    Affinity and Kinetic Characterization of 83945-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83945-5-RR against Human EOMES were performed. The affinity constant is below 1 pM.

EOMES/TBR2 Recombinant antibody, PBS Only

EOMES/TBR2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 83945-5-PBS

产品说明书

CloneNo. 241036D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EOMES, TBR2, 241036D9, Eomesodermin homolog, T box brain protein 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83945-5-PBS targets EOMES/TBR2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 EOMES/TBR2 fusion protein Ag27634 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eomesodermin homolog (Xenopus laevis)
别名 EOMES, TBR2, 241036D9, Eomesodermin homolog, T box brain protein 2
计算分子量 73 aa
观测分子量 67 kDa
GenBank蛋白编号NM_001278182
基因名称 EOMES
Gene ID (NCBI) 8320
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO95936
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EOMES/TRB2 (also named Eomesodermin/Tbr2) encode T-box transcription factor expressed highly in the intermediate progenitor stage of the developing neuron. During migration of the neurons, radial glia divide and migrate towards the surface of the cerebral cortex, there are three stages of cellular development: radial glia, intermediate progenitors, and postmitotic projection neurons. Radial glia express Pax6, while intermediate progenitor cells express Eomesodermin/Tbr2, and postmitotic projection neurons express Tbr1 (PMID:15634788; PMID:16320258). This process also called neurogenesis, and demonstrated that Eomesodermin/Tbr2 has been implicated in neurodevelopment. According to the mouse model, several articles showed that EOMES/TRB2 KO mice having proliferating cells decreased and having smaller upper cortical layers and a smaller sub ventricular zone in the brain (PMID:18940588). What's more, Eomesodermin/Tbr2 has also been found participating in immune response (PMID:20713880).