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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 21010-1-AP

    WB analysis using 21010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ENTPD8 Polyclonal antibody

ENTPD8 Polyclonal Antibody for WB, ELISA
Cat No. 21010-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

WB, ELISA

别名

E NTPDase 8, ENTPD8, GLSR2492, NTPDase 8, NTPDase8, UNQ2492

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in37°C incubated HepG2 cells, mouse liver tissue, rat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21010-1-AP targets ENTPD8 in WB, ELISA applications and shows reactivity with human, rat, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat, mouse
免疫原

CatNo: Ag15224

Product name: Recombinant human ENTPD8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 131-350 aa of BC141810

Sequence: LSRKNSSQARDIFAAVTQVLGRSPVDFWGAELLAGQAEGAFGWITVNYGLGTLVKYSFTGEWIQPPEEMLVGALDMGGASTQITFVPGGPILDKSTQADFRLYGSDYSVYTHSYLCFGRDQMLSRLLVGLVQSRPAALLRHPCYLSGYQTTLALGPLYESPCVHATPPLSLPQNLTVEGTGNPGACVSAIRELFNFSSCQGQEDCAFDGVYQPPLRGQFY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ectonucleoside triphosphate diphosphohydrolase 8
别名 E NTPDase 8, ENTPD8, GLSR2492, NTPDase 8, NTPDase8, UNQ2492
计算分子量 495 aa, 54 kDa
观测分子量 70 kDa, 54 kDa
GenBank蛋白编号BC141810
基因名称 ENTPD8
Gene ID (NCBI) 377841
RRIDAB_10755288
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5MY95
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENTPD8 (Ectonucleoside triphosphate diphosphohydrolase 8) is also named as E-NTPDase 8, NTPDase8 and belongs to the GDA1/CD39 NTPase family. NTPDase8 is the canalicular ecto-ATPase/ATPDase and is responsible for the main hepatic NTP-Dase activity(PMID:17095758). The estimated molecular mass of expressed human ENTPD8 is 70-85 kDa which is N-glycosylated and after deglycosylation, it has a nearly identical molecular mass, 54 kDa(PMID:16752921;7781224). It has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 49 kDa. This antibody is specific to ENTPD8.

实验方案

Product Specific Protocols
WB protocol for ENTPD8 antibody 21010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols