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ENTPD8 Polyclonal antibody

ENTPD8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 21010-1-AP

Print datasheet

Synonyms

E NTPDase 8, ENTPD8, GLSR2492, NTPDase 8, NTPDase8, UNQ2492

验证数据展示

  • WB analysis using 21010-1-AP

    WB analysis using 21010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected in37°C incubated HepG2 cells, mouse liver tissue, rat liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21010-1-AP targets ENTPD8 in WB, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, ELISA
Tested Reactivity human, rat, mouse
Immunogen ENTPD8 fusion protein Ag15224 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ectonucleoside triphosphate diphosphohydrolase 8
Synonyms E NTPDase 8, ENTPD8, GLSR2492, NTPDase 8, NTPDase8, UNQ2492
Calculated Molecular Weight 495 aa, 54 kDa
Observed Molecular Weight 70 kDa, 54 kDa
GenBank Accession NumberBC141810
Gene Symbol ENTPD8
Gene ID (NCBI) 377841
RRIDAB_10755288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5MY95
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENTPD8 (Ectonucleoside triphosphate diphosphohydrolase 8) is also named as E-NTPDase 8, NTPDase8 and belongs to the GDA1/CD39 NTPase family. NTPDase8 is the canalicular ecto-ATPase/ATPDase and is responsible for the main hepatic NTP-Dase activity(PMID:17095758). The estimated molecular mass of expressed human ENTPD8 is 70-85 kDa which is N-glycosylated and after deglycosylation, it has a nearly identical molecular mass, 54 kDa(PMID:16752921;7781224). It has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 49 kDa. This antibody is specific to ENTPD8.

实验方案

Product Specific Protocols
WB protocol for ENTPD8 antibody 21010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols