验证数据展示
经过测试的应用
| Positive WB detected in | 37°C incubated HepG2 cells, mouse liver tissue, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21010-1-AP targets ENTPD8 in WB, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat, mouse |
| 免疫原 | ENTPD8 fusion protein Ag15224 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ectonucleoside triphosphate diphosphohydrolase 8 |
| 别名 | E NTPDase 8, ENTPD8, GLSR2492, NTPDase 8, NTPDase8, UNQ2492 |
| 计算分子量 | 495 aa, 54 kDa |
| 观测分子量 | 70 kDa, 54 kDa |
| GenBank蛋白编号 | BC141810 |
| 基因名称 | ENTPD8 |
| Gene ID (NCBI) | 377841 |
| RRID | AB_10755288 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5MY95 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ENTPD8 (Ectonucleoside triphosphate diphosphohydrolase 8) is also named as E-NTPDase 8, NTPDase8 and belongs to the GDA1/CD39 NTPase family. NTPDase8 is the canalicular ecto-ATPase/ATPDase and is responsible for the main hepatic NTP-Dase activity(PMID:17095758). The estimated molecular mass of expressed human ENTPD8 is 70-85 kDa which is N-glycosylated and after deglycosylation, it has a nearly identical molecular mass, 54 kDa(PMID:16752921;7781224). It has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 49 kDa. This antibody is specific to ENTPD8.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ENTPD8 antibody 21010-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
