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验证数据展示

  • WB analysis using 31022-1-AP (same clone as 31022-1-PBS)

    WB analysis using 31022-1-AP (same clone as 31022-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31022-1-AP (ENOSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31022-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 31022-1-AP (same clone as 31022-1-PBS)

    IP experiment of MCF-7 using 31022-1-AP (same clone as 31022-1-PBS)

    IP result of anti-ENOSF1 (IP:31022-1-AP, 4ug; Detection:31022-1-AP 1:2000) with MCF-7 cells lysate 1600 ug. This data was developed using the same antibody clone with 31022-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 31022-1-AP (same clone as 31022-1-PBS)

    FC experiment of HeLa using 31022-1-AP (same clone as 31022-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ENOSF1 Polyclonal antibody (31022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 31022-1-PBS in a different storage buffer formulation.

ENOSF1 Polyclonal antibody, PBS Only

ENOSF1 Polyclonal Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 31022-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), IP, Indirect ELISA

别名

EC:4.2.1.68, enolase superfamily member 1, L-fuconate dehydratase, rTS, TYMSAS

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31022-1-PBS targets ENOSF1 in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34665

Product name: Recombinant human ENOSF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-443 aa of BC001285

Sequence: MVRGRISRLSVRDVRFPTSLGGHGADAMHTDPDYSAAYVVIETDAEDGIKGCGITFTLGKGTEVVVCAVNALAHHVLNKDLKDIVGDFRGFYRQLTSDGQLRWIGPEKGVVHLATAAVLNAVWDLWAKQEGKPVWKLLVDMDPRMLVSCIDFRYITDVLTEEDALEILQKGQIGKKEREKQMLAQGYPAYTTSCAWLGYSDDTLKQLCAQALKDGWTRFKVKVGADLQDDMRRCQIIRDMIGPEKTLMMDANQRWDVPEAVEWMSKLAKFKPLWIEEPTSPDDILGHATISKALVPLGIGIATGEQCHNRVIFKQLLQAKALQFLQIDSCRLGSVNENLSVLLMAKKFEIPVCPHAGGVGLCELVQHLIIFDYISVSASLENRVCEYVDHLHEHFKYPVMIQRASYMPPKDPGYSTEMKEESVKKHQYPDGEVWKKLLPAQEN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enolase superfamily member 1
别名 EC:4.2.1.68, enolase superfamily member 1, L-fuconate dehydratase, rTS, TYMSAS
计算分子量 50 kDa
观测分子量 49 kDa
GenBank蛋白编号BC001285
基因名称 ENOSF1
Gene ID (NCBI) 55556
RRIDAB_3669818
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ7L5Y1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).

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