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ENOSF1 Polyclonal antibody

ENOSF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 31022-1-AP

Print datasheet

Synonyms

enolase superfamily member 1, ENOSF1, HSRTSBETA, RTS, TYMSAS

验证数据展示

  • WB analysis using 31022-1-AP

    WB analysis using 31022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31022-1-AP (ENOSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31022-1-AP targets ENOSF1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen ENOSF1 fusion protein Ag34665 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enolase superfamily member 1
Synonyms enolase superfamily member 1, ENOSF1, HSRTSBETA, RTS, TYMSAS
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC001285
Gene Symbol ENOSF1
Gene ID (NCBI) 55556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ7L5Y1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).

实验方案

Product Specific Protocols
WB protocol for ENOSF1 antibody 31022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols