验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HL-60 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11763-1-AP targets ENOPH1 in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2360 Product name: Recombinant human ENOPH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-115 aa of BC001317 Sequence: MKVYIYSSGSVEAQKLLFGHSTEGDILELVDGHFDTKIGHKVESESYRKIADSIGCSTNNILFLTDVTREASAAEEADVHVAVVVRPGNAGLTDDEKTYYSLITSFSELYLPSST 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | enolase-phosphatase 1 |
| 别名 | E1, enolase phosphatase 1, Enolase phosphatase E1, ENOPH1, MASA, MASA homolog, MST145 |
| 计算分子量 | 13 kDa / 29 kDa |
| 观测分子量 | 29-35 kDa |
| GenBank蛋白编号 | BC001317 |
| 基因名称 | ENOPH1 |
| Gene ID (NCBI) | 58478 |
| RRID | AB_10794441 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHY7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ENOPH1, also named as MASA and MSTP145, belongs to the HAD-like hydrolase superfamily and MasA/MtnC family. ENOPH1 is a newly identified enzyme involved in L-methionine biosynthesis, which is associated with anxiety and depression. Studies have shown that ENOPH1 plays a crucial role in promoting the proliferation and migration of glioma cells (PMID: 32654229). ENOPH1 has 2 isoforms with the molecular mass of 13 and 29 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ENOPH1 antibody 11763-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Mol Neurosci Cerebral Microvascular Endothelial Cell Apoptosis after Ischemia: Role of Enolase-Phosphatase 1 Activation and Aci-Reductone Dioxygenase 1 Translocation.
| ||
Oncol Rep Enolase-phosphatase 1 as a novel potential malignant glioma indicator promotes cell proliferation and migration.
|
