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CoraLite®594-conjugated ENO1 Monoclonal antibody

ENO1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D6E8

Cat No : CL594-67187

Print datasheet

Synonyms

Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH

验证数据展示

  • IF Staining of HeLa using CL594-67187

    IF Staining of HeLa using CL594-67187

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated ENO1 antibody (CL594-67187, Clone: 1D6E8 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67187-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67187 targets ENO1 in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF/ICC
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen ENO1 fusion protein Ag17518 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name enolase 1, (alpha)
Synonyms Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC015641
Gene Symbol ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
RRIDAB_2920094
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP06733
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656)

实验方案

Product Specific Protocols
IF protocol for CL594 ENO1 antibody CL594-67187Download protocol
Standard Protocols
Click here to view our Standard Protocols