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ENO1/2/3 Recombinant antibody

ENO1/2/3 Recombinant Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

6E24

Cat No : 81504-1-RR

Print datasheet

Synonyms

Alpha enolase, C myc promoter binding protein, ENO1, ENO1/2/3, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH

验证数据展示

  • WB analysis using 81504-1-RR

    WB analysis using 81504-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81504-1-RR (ENO1/2/3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 81504-1-RR

    IHC staining of mouse brain using 81504-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81504-1-RR (ENO1/2/3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ENO1 antibody included
  • IF Staining of HepG2 using 81504-1-RR

    IF Staining of HepG2 using 81504-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ENO1/2/3 antibody (81504-1-RR, Clone: 6E24 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, PC-3 cells, MCF-7 cells, Jurkat cells, mouse brain tissue, rat brain tissue, pig brain tissue
Positive IHC detected inmouse brain tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81504-1-RR targets ENO1/2/3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen ENO1/2/3 fusion protein Ag1692 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name enolase 1, (alpha)
Synonyms Alpha enolase, C myc promoter binding protein, ENO1, ENO1/2/3, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC015641
Gene Symbol ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06733
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enolase (ENO), also known as 2-phospho-D-glycerate hydrolase, is a metalloenzyme that catalyzes the dehydration of 2-phospho-D-glycerate into phosphoenolpyruvate in the glycolytic pathway. There are three different ENO isoforms in higher eukaryotes, including ENO1 or α-ENO, ENO2 or γ-ENO, and ENO3 or β-ENO. Furthermore, the expression of the different ENO isoforms is tissue specificity. ENO1 is expressed in almost all kinds of tissue, whereas ENO3 is restricted to muscle tissues and ENO2 is described as a neuron- and neuroendocrine tissue-specific enolase. ENO1 serves multifunctional roles in physiological and pathological processes. (PMID: 32256815). This antibody can recognize ENO1, ENO2, and ENO3.

实验方案

Product Specific Protocols
WB protocol for ENO1/2/3 antibody 81504-1-RRDownload protocol
IHC protocol for ENO1/2/3 antibody 81504-1-RRDownload protocol
IF protocol for ENO1/2/3 antibody 81504-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols