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验证数据展示

  • IHC staining of mouse spleen using 31784-1-AP (same clone as 31784-1-PBS)

    IHC staining of mouse spleen using 31784-1-AP (same clone as 31784-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 31784-1-AP (same clone as 31784-1-PBS)

    IF Staining of U-251 using 31784-1-AP (same clone as 31784-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using ENGASE antibody (31784-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

ENGASE Polyclonal antibody, PBS Only

ENGASE Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 31784-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

EC:3.2.1.96, Cytosolic endo-beta-N-acetylglucosaminidase

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31784-1-PBS targets ENGASE in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34633

Product name: Recombinant human ENGASE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-200 aa of NM_001042573

Sequence: IKDEEEETVFREVVSFSPDPLPVRYYDKDTTKPISFYLSSLEELLAWKPRLEDGFNVALEPLACRQPPLSSQRPRTLLCHDMMGGYLDDRFIQGSVVQTPYAFYHWQCIDVFVYFSHHTVTIPPVGWTNTAHRHGVCVLGTFITEWNEGGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 endo-beta-N-acetylglucosaminidase
别名 EC:3.2.1.96, Cytosolic endo-beta-N-acetylglucosaminidase
计算分子量84 kDa
GenBank蛋白编号NM_001042573
基因名称 ENGASE
Gene ID (NCBI) 64772
RRIDAB_3670113
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8NFI3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Endo-β-N-acetylglucosaminidase (ENGASE) is an enzyme that belongs to glycoside hydrolase (GH) families 18 and 85, and is widely distributed in bacteria, fungi, and higher-order species. ENGASE hydrolyzes the β1-4 linkage of the N, N-diacetylchitobiose (GlcNAcβ1-4GlcNAc) region of the N-linked glycans present on glycoproteins (PMID: 36435009). ENGASE is widely expressed, with higher expression in thymus and spleen (Refer to Uniprot).

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