验证数据展示
经过测试的应用
Positive WB detected in | NIH/3T3 cells, HeLa cells, mouse heart tissue, mouse liver tissue, rat liver tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
86122-1-RR targets ENDOG in applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | ENDOG fusion protein Ag17739 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | endonuclease G |
别名 | EC:3.1.30.-, Endo G, Endonuclease G |
计算分子量 | 297 aa, 33 kDa |
观测分子量 | 25-30 kDa |
GenBank蛋白编号 | BC016351 |
基因名称 | ENDOG |
Gene ID (NCBI) | 2021 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q14249 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for ENDOG antibody 86122-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |