ENDOG Recombinant antibody

ENDOG Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 86122-1-RR

产品说明书

CloneNo. 250804D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

EC:3.1.30.-, Endo G, Endonuclease G

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells, mouse heart tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86122-1-RR targets ENDOG in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ENDOG fusion protein Ag17739 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 endonuclease G
别名 EC:3.1.30.-, Endo G, Endonuclease G
计算分子量 297 aa, 33 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC016351
基因名称 ENDOG
Gene ID (NCBI) 2021
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14249
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.

实验方案

Product Specific Protocols
WB protocol for ENDOG antibody 86122-1-RRDownload protocol
IHC protocol for ENDOG antibody 86122-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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