ENDOG Recombinant antibody

ENDOG Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86122-1-RR

产品说明书

CloneNo. 250804D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

EC:3.1.30.-, Endo G, Endonuclease G

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells, mouse heart tissue, mouse liver tissue, rat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86122-1-RR targets ENDOG in applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ENDOG fusion protein Ag17739 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 endonuclease G
别名 EC:3.1.30.-, Endo G, Endonuclease G
计算分子量 297 aa, 33 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC016351
基因名称 ENDOG
Gene ID (NCBI) 2021
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14249
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.

实验方案

Product Specific Protocols
WB protocol for ENDOG antibody 86122-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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