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验证数据展示

  • WB analysis using 86122-1-RR (same clone as 86122-1-PBS)

    WB analysis using 86122-1-RR (same clone as 86122-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86122-1-RR (ENDOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86122-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02519-1

    Sandwich ELISA standard curve of MP02519-1

    Sandwich ELISA standard curve of MP02519-1, Human ENDOG Recombinant Matched Antibody Pair - PBS only. 86122-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17739. 86122-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL.

  • Sample test of MP02519-1

    Sample test of MP02519-1

    The mean ENDOG concentration was determined to be 1,103.00 ng/mL in Ramos cell extract based on a 1.40 mg/mL extract load, 1,271.00 ng/mL in HepG2 cell extract based on a 1.3 mg/mL extract load and 967.00 ng/mL in HL-60 cell extract based on a 3.7 mg/mL extract load.

  • IHC staining of mouse kidney using 86122-1-RR (same clone as 86122-1-PBS)

    IHC staining of mouse kidney using 86122-1-RR (same clone as 86122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86122-1-RR (ENDOG antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86122-1-PBS in a different storage buffer formulation.

ENDOG Recombinant antibody, PBS Only (Capture)

ENDOG Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 86122-1-PBS

产品说明书

CloneNo. 250804D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

别名

EC:3.1.30.-, Endo G, Endonuclease G

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86122-1-PBS targets ENDOG as part of a matched antibody pair:

MP02519-1: 86122-1-PBS capture and 86122-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 ENDOG fusion protein Ag17739 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 endonuclease G
别名 EC:3.1.30.-, Endo G, Endonuclease G
计算分子量 297 aa, 33 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC016351
基因名称 ENDOG
Gene ID (NCBI) 2021
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14249
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.