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EMD Recombinant antibody

EMD Recombinant Antibody for IF/ICC, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230089C4

Cat No : 82888-2-RR

Print datasheet

Synonyms

EDMD, EMD, emerin, LEMD5, STA

验证数据展示

  • FC experiment of HeLa using 82888-2-RR

    FC experiment of HeLa using 82888-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human EMD (82888-2-RR, Clone:230089C4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human thyroid cancer using 82888-2-RR

    IHC staining of human thyroid cancer using 82888-2-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82888-2-RR (EMD antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 82888-2-RR

    IHC staining of human thyroid cancer using 82888-2-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82888-2-RR (EMD antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 82888-2-RR

    IF Staining of HeLa using 82888-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EMD antibody (82888-2-RR, Clone: 230089C4 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:150-1:600
Flow Cytometry (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82888-2-RR targets EMD in IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC, IHC, ELISA
Tested Reactivity Human
Immunogen EMD fusion protein Ag0236 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name emerin
Synonyms EDMD, EMD, emerin, LEMD5, STA
Calculated Molecular Weight 34 kDa
GenBank Accession NumberBC000738
Gene Symbol EMD
Gene ID (NCBI) 2010
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50402
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for EMD antibody 82888-2-RRDownload protocol
IF protocol for EMD antibody 82888-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols