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EMCN Polyclonal antibody

EMCN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11828-1-AP

Print datasheet

Synonyms

EMCN, EMCN2, endomucin, Endomucin 2, Gastric cancer antigen Ga34, MUC 14, MUC14, Mucin 14

验证数据展示

  • WB analysis using 11828-1-AP

    WB analysis using 11828-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11828-1-AP (EMCN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 11828-1-AP

    IHC staining of mouse kidney using 11828-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11828-1-AP (EMCN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 11828-1-AP

    IHC staining of mouse kidney using 11828-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11828-1-AP (EMCN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse lung tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11828-1-AP targets EMCN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen EMCN fusion protein Ag2396 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name endomucin
Synonyms EMCN, EMCN2, endomucin, Endomucin 2, Gastric cancer antigen Ga34, MUC 14, MUC14, Mucin 14
Calculated Molecular Weight 19 kDa, 27 kDa
Observed Molecular Weight85 kDa
GenBank Accession NumberBC017781
Gene Symbol EMCN
Gene ID (NCBI) 51705
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULC0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endomucin (EMCN), also known as Endomucin 2 or Mucin 14, is a type I integral membrane glycoprotein that is endothelial-specific. EMCN is expressed on the surface of capillaries and venous and is highly expressed in highly vascularized tissues such as heart, kidney and lung (PMID: 29215001). Endomucin plays an important role in cell interaction, molecular cell signaling, angiogenesis and cell migration (PMID: 33532708). Since it is glycosylated, the apparent molecular weight of EMCN could be variable, ranging from 80 kDa to 120 kDa.

实验方案

Product Specific Protocols
WB protocol for EMCN antibody 11828-1-APDownload protocol
IHC protocol for EMCN antibody 11828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols