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验证数据展示

  • WB analysis using 21070-1-AP

    WB analysis using 21070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21070-1-AP (ELOF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 21070-1-AP

    WB analysis using 21070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21070-1-AP (ELOF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 21070-1-AP

    IF Staining of A549 using 21070-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ELOF1 antibody (21070-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of SKOV-3 using 21070-1-AP

    IF Staining of SKOV-3 using 21070-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using ELOF1 antibody (21070-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ELOF1 Polyclonal antibody

ELOF1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21070-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ELF1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, HEK-293 cells, rat testis tissue
Positive IF/ICC detected inA549 cells, SKOV-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21070-1-AP targets ELOF1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15213

Product name: Recombinant human ELOF1 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-83 aa of BC007516

Sequence: MGRRKSKRKPPPKKKMTGTLETQFTCPFCNHEKSCDVKMDRARNTGVISCTVCLEEFQTPITYLSEPVDVYSDWIDACEAANQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elongation factor 1 homolog (S. cerevisiae)
别名 ELF1
计算分子量9.5 kDa
观测分子量 9 kDa
GenBank蛋白编号BC007516
基因名称 ELOF1
Gene ID (NCBI) 84337
RRIDAB_10693632
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60002
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELOF1 (elongation factor 1 homologue) is a small (83 aa), evolutionarily conserved, RNAPII-associated transcription elongation factor that is also a core component of the transcription-coupled DNA repair machinery with an additional role in preventing DNA damage during DNA replication (PMID: 34108663). Loss of ELOF1 strongly compromises AID-mediated deamination, SHM, and CSR, and that this is accompanied by a decrease in parameters of RNAPII pausing/stalling and in RNAPII RNA synthesis without a decrease in levels of S2P-RNAPII (PMID: 39386505).

实验方案

Product Specific Protocols
WB protocol for ELOF1 antibody 21070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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