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验证数据展示

  • WB analysis using 21070-1-AP

    WB analysis using 21070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21070-1-AP (ELOF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 21070-1-AP

    WB analysis using 21070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21070-1-AP (ELOF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ELOF1 Polyclonal antibody

ELOF1 Polyclonal Antibody for WB, ELISA
Cat No. 21070-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

ELF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, HEK-293 cells, rat testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21070-1-AP targets ELOF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ELOF1 fusion protein Ag15213 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elongation factor 1 homolog (S. cerevisiae)
别名 ELF1
计算分子量9.5 kDa
观测分子量 9 kDa
GenBank蛋白编号BC007516
基因名称 ELOF1
Gene ID (NCBI) 84337
RRIDAB_10693632
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60002
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELOF1 (elongation factor 1 homologue) is a small (83 aa), evolutionarily conserved, RNAPII-associated transcription elongation factor that is also a core component of the transcription-coupled DNA repair machinery with an additional role in preventing DNA damage during DNA replication (PMID: 34108663). Loss of ELOF1 strongly compromises AID-mediated deamination, SHM, and CSR, and that this is accompanied by a decrease in parameters of RNAPII pausing/stalling and in RNAPII RNA synthesis without a decrease in levels of S2P-RNAPII (PMID: 39386505).

实验方案

Product Specific Protocols
WB protocol for ELOF1 antibody 21070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols