验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | mouse spleen tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | K-562 cells, Jurkat cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26974-1-AP targets ELMO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | ELMO1 fusion protein Ag25635 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | engulfment and cell motility 1 |
别名 | ELMO 1, engulfment and cell motility 1 |
观测分子量 | 80~84 kDa |
GenBank蛋白编号 | BC114341 |
基因名称 | ELMO1 |
Gene ID (NCBI) | 9844 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q92556 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELMO1 (Engulfment and cell motility protein 1) is a cytoplasmic adapter protein that physically associates with members of the Dock-A family of Rac-GEFs, of which Dock1 and Dock2 are the best characterized (PMID: 24821968). ELMO1 is expressed in platelets and negatively regulates integrin-mediated platelet spreading. Upregulated ELMO1 expression is associated with a poor prognosis in hepatocellular carcinoma (HCC), as well as increased cell growth, invasion, migration, angiogenesis and EMT in vitro and in vivo (PMID: 31324602).
实验方案
Product Specific Protocols | |
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WB protocol for ELMO1 antibody 26974-1-AP | Download protocol |
IHC protocol for ELMO1 antibody 26974-1-AP | Download protocol |
IF protocol for ELMO1 antibody 26974-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |