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验证数据展示

  • WB analysis using 26974-1-AP

    WB analysis using 26974-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26974-1-AP (ELMO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 26974-1-AP

    IHC staining of human placenta using 26974-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 26974-1-AP

    IHC staining of mouse spleen using 26974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 26974-1-AP

    IHC staining of mouse spleen using 26974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 26974-1-AP

    IF Staining of Jurkat using 26974-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ELMO1 antibody (26974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of K-562 using 26974-1-AP

    IF Staining of K-562 using 26974-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ELMO1 antibody (26974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ELMO1 Polyclonal antibody

ELMO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26974-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ELMO 1, engulfment and cell motility 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse spleen tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26974-1-AP targets ELMO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25635

Product name: Recombinant human ELMO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 76-158 aa of BC114341

Sequence: RLTTSPAQNAQQLHERIQSSSMDAKLEALKDLASLSRDVTFAQEFINLDGISLLTQMVESGTERYQKLQKIMKPCFGDMLSFT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 engulfment and cell motility 1
别名 ELMO 1, engulfment and cell motility 1
观测分子量80~84 kDa
GenBank蛋白编号BC114341
基因名称 ELMO1
Gene ID (NCBI) 9844
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92556
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELMO1 (Engulfment and cell motility protein 1) is a cytoplasmic adapter protein that physically associates with members of the Dock-A family of Rac-GEFs, of which Dock1 and Dock2 are the best characterized (PMID: 24821968). ELMO1 is expressed in platelets and negatively regulates integrin-mediated platelet spreading. Upregulated ELMO1 expression is associated with a poor prognosis in hepatocellular carcinoma (HCC), as well as increased cell growth, invasion, migration, angiogenesis and EMT in vitro and in vivo (PMID: 31324602).

实验方案

Product Specific Protocols
IF protocol for ELMO1 antibody 26974-1-APDownload protocol
IHC protocol for ELMO1 antibody 26974-1-APDownload protocol
WB protocol for ELMO1 antibody 26974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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