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验证数据展示

  • WB analysis of HeLa using 32800-1-AP

    WB analysis of HeLa using 32800-1-AP

    WB result of ELL2 antibody (32800-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELL2 transfected HeLa cells.

  • WB analysis using 32800-1-AP

    WB analysis using 32800-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32800-1-AP (ELL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

ELL2 Polyclonal antibody

ELL2 Polyclonal Antibody for WB, ELISA
Cat No. 32800-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

ELL 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32800-1-AP targets ELL2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag39224

Product name: Recombinant human ELL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 393-515 aa of BC028412

Sequence: NSNSNSPSTPEGRGTQDLPVDSFSQNDSIYEDQQDKYTSRTSLETLPPGSVLLKCPKPMEENHSMSHKKSKKKSKKHKEKDQIKKHDIETIEEKEEDLKREEEIAKLNNSSPNSSGGVKEDCT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elongation factor, RNA polymerase II, 2
别名 ELL 2
计算分子量 640 aa, 72 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC028412
基因名称 ELL2
Gene ID (NCBI) 22936
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00472
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELL2 (Elongation Factor for RNA Polymerase II 2) is a key transcriptional elongation factor that regulates RNA polymerase II (Pol II) during mRNA synthesis (PMID: 34265249). It belongs to the ELL gene family, which includes ELL1, ELL2, and ELL3, and plays a crucial role in controlling the elongation phase of transcription (PMID: 9108030). ELL2 is an androgen-responsive gene that is expressed by prostate epithelial cells and is frequently down-regulated in prostate cancer (PMID: 29179998). The predicted molecular weight of the ELL2 protein is 72 kDa, but due to post-translational modifications, its molecular weight is approximately 90 kDa. (PMID: 39582094, PMID: 34948391)

实验方案

Product Specific Protocols
IF protocol for ELL2 antibody 32800-1-APDownload protocol
WB protocol for ELL2 antibody 32800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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