验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells, mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
14666-1-AP targets ELK4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ELK4 fusion protein Ag6273 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ELK4, ETS-domain protein (SRF accessory protein 1) |
| 别名 | ELK4, SAP 1, SAP1, SRF accessory protein 1 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC063676 |
| 基因名称 | ELK4 |
| Gene ID (NCBI) | 2005 |
| RRID | AB_2262305 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28324 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELK4, also named as SAP1, SAP-1 and SAP1, belongs to the ETS family. It forms a ternary complex with the serum response factor (SRF). ELK4 requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously. It is androgen regulated, involved in promoting cell growth, and highly expressed in a subset of prostate cancer. (PMID:19293179 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ELK4 antibody 14666-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol tRNA-Derived Fragment tRF-Glu-TTC-027 Regulates the Progression of Gastric Carcinoma via MAPK Signaling Pathway | ||
J Pers Med Exosomes from Microvascular Endothelial Cells under Mechanical Unloading Inhibit Osteogenic Differentiation via miR-92b-3p/ELK4 Axis | ||
Brain Res Bull ELK4 ameliorates cognitive impairment and neuroinflammation induced by obstructive sleep apnea |
