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验证数据展示

  • WB analysis using 83468-6-RR (same clone as 83468-6-PBS)

    WB analysis using 83468-6-RR (same clone as 83468-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83468-6-RR (ELAC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83468-6-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 83468-6-RR (same clone as 83468-6-PBS)

    WB analysis of MCF-7 using 83468-6-RR (same clone as 83468-6-PBS)

    WB result of ELAC2 antibody (83468-6-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELAC2 transfected MCF-7 cells. This data was developed using the same antibody clone with 83468-6-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83468-6-RR (same clone as 83468-6-PBS)

    FC experiment of A431 using 83468-6-RR (same clone as 83468-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ELAC2 Recombinant antibody (83468-6-RR, Clone:240487B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83468-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83468-6-RR

    Affinity and Kinetic Characterization of 83468-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83468-6-RR against Human ELAC2 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

ELAC2 Recombinant monoclonal antibody, PBS Only

ELAC2 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83468-6-PBS

产品说明书

CloneNo. 240487B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

240487B6, EC:3.1.26.11, elaC homolog 2 (E. coli), ElaC homolog protein 2, ELC2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ELAC2 Ready-To-Use IHC Kit. ELAC2 primary antibody included.

产品信息

83468-6-PBS targets ELAC2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0105

Product name: Recombinant human ELAC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 86-385 aa of BC004158

Sequence: NCGEGVQRLMQEHKLKVARLDNIFLTRMHWSNVGGLSGMILTLKETGLPKCVLSGPPQLEKYLEAIKIFSGPLKGIELAVRPHSAPEYEDETMTVYQIPIHSEQRRGKHQPWQSPERPLSRLSPERSSDSELNENEPHLPHGVSQRRGVRDSSLVVAFICKLHLKRGNFLVLKAKEMGLPVGTAAIAPIIAAVKDGKSITHEGREILAEELCTPPDPGAAFVVVECPDESFIQPICENATFQRYQGKADAPVALVVHMAPASVLVDSRYQQWMERFGPDTQHLVLNENCASVHNLRSHKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 elaC homolog 2 (E. coli)
别名 240487B6, EC:3.1.26.11, elaC homolog 2 (E. coli), ElaC homolog protein 2, ELC2
计算分子量 92 kDa
观测分子量 97 kDa
GenBank蛋白编号BC004158
基因名称 ELAC2
Gene ID (NCBI) 60528
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BQ52
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).

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