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验证数据展示

  • IHC staining of human liver cancer using 26639-1-AP

    IHC staining of human liver cancer using 26639-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26639-1-AP (ELAC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 26639-1-AP

    IHC staining of human liver cancer using 26639-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26639-1-AP (ELAC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse liver using 26639-1-AP

    WB analysis of mouse liver using 26639-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26639-1-AP (ELAC1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ELAC1 Polyclonal antibody

ELAC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26639-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

D29, Deleted in Ma29, elaC homolog 1 (E. coli), ElaC homolog protein 1, ELAC1, Ribonuclease Z 1, RNase Z 1, tRNA 3 endonuclease 1, tRNase Z 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26639-1-AP targets ELAC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag23939

Product name: Recombinant human ELAC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-271 aa of BC014624

Sequence: MSMDVTFLGTGAAYPSPTRGASAVVLRCEGECWLFDCGEGTQTQLMKSQLKAGRITKIFITHLHGDHFFGLPGLLCTISLQSGSMVSKQPIEIYGPVGLRDFIWRTMELSHTELVFHYVVHELVPTADQCPAEELKEFAHVNRADSPPKEEQGRTILLDSEENSYLLFDDEQFVVKAFRLFHRIPSFGFSVVEKKRPGKLNAQKLKDLGVPPGPAYGKLKNGISVVLENGVTISPQDVLKKPIVGRKICILGDCSGVVGDGGVKLCFEADL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elaC homolog 1 (E. coli)
别名 D29, Deleted in Ma29, elaC homolog 1 (E. coli), ElaC homolog protein 1, ELAC1, Ribonuclease Z 1, RNase Z 1, tRNA 3 endonuclease 1, tRNase Z 1
观测分子量 45-48 kDa
GenBank蛋白编号BC014624
基因名称 ELAC1
Gene ID (NCBI) 55520
RRIDAB_2880586
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H777
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ELAC1 antibody 26639-1-APDownload protocol
WB protocol for ELAC1 antibody 26639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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