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验证数据展示

  • WB analysis using 11281-1-AP (same clone as 11281-1-PBS)

    WB analysis using 11281-1-AP (same clone as 11281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 11281-1-AP (same clone as 11281-1-PBS)

    IHC staining of human gliomas using 11281-1-AP (same clone as 11281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11281-1-AP (EIF4G3 antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

    IP experiment of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

    IP result of anti-EIF4G3 (IP:11281-1-AP, 4ug; Detection:11281-1-AP 1:500) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

    IF Staining of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF4G3 antibody (11281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

EIF4G3 Polyclonal antibody, PBS Only

EIF4G3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11281-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

eIF4GII, eIF-4-gamma II, eIF-4-gamma 3, eIF-4G 3, eIF4G 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11281-1-PBS targets EIF4G3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1811

Product name: Recombinant human EIF4G3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 197-515 aa of BC030578

Sequence: SQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDGDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPETSNEC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4 gamma, 3
别名 eIF4GII, eIF-4-gamma II, eIF-4-gamma 3, eIF-4G 3, eIF4G 3
计算分子量 177 kDa
观测分子量 70 kDa, 250 kDa
GenBank蛋白编号BC030578
基因名称 EIF4G3
Gene ID (NCBI) 8672
RRIDAB_2877754
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43432
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein migrated at approximately 70 kDa and/or 250 kDa in SDS-PAGE.

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