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EIF4G3 Polyclonal antibody

EIF4G3 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11281-1-AP

Print datasheet

Synonyms

eIF 4 gamma 3, eIF 4 gamma II, eIF 4G 3, eIF4G 3, EIF4G3, eIF4GII

验证数据展示

  • WB analysis using 11281-1-AP

    WB analysis using 11281-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 11281-1-AP

    IHC staining of human gliomas using 11281-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11281-1-AP (EIF4G3 antibody) at dilution of 1:10 (under 10x lens).

  • IP experiment of HeLa using 11281-1-AP

    IP experiment of HeLa using 11281-1-AP

    IP result of anti-EIF4G3 (IP:11281-1-AP, 4ug; Detection:11281-1-AP 1:500) with HeLa cells lysate 4000ug.

  • IF Staining of HeLa using 11281-1-AP

    IF Staining of HeLa using 11281-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11281-1-AP (EIF4G3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11281-1-AP targets EIF4G3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen EIF4G3 fusion protein Ag1811 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4 gamma, 3
Synonyms eIF 4 gamma 3, eIF 4 gamma II, eIF 4G 3, eIF4G 3, EIF4G3, eIF4GII
Calculated Molecular Weight 177 kDa
Observed Molecular Weight 70 kDa, 250 kDa
GenBank Accession NumberBC030578
Gene Symbol EIF4G3
Gene ID (NCBI) 8672
RRIDAB_2877754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43432
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein migrated at approximately 70 kDa and/or 250 kDa in SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for EIF4G3 antibody 11281-1-APDownload protocol
IHC protocol for EIF4G3 antibody 11281-1-APDownload protocol
IF protocol for EIF4G3 antibody 11281-1-APDownload protocol
IP protocol for EIF4G3 antibody 11281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Jpn J Clin Oncol

Identification of distinct genomic features reveals frequent somatic AHNAK and PTEN mutations predominantly in primary malignant melanoma presenting in the ureter.

Authors - Yan Huang
View Article
humanWB

Cancer Res

Fatty Acid Oxidation Mediated by Malonyl-CoA Decarboxylase Represses Renal Cell Carcinoma Progression

Authors - Lijie Zhou
View Article