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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 67199-1-Ig (same clone as 67199-1-PBS)

    WB analysis using 67199-1-Ig (same clone as 67199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67199-1-Ig (EIF4G1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67199-1-Ig (same clone as 67199-1-PBS)

    IF Staining of HepG2 using 67199-1-Ig (same clone as 67199-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

    IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF4G1 antibody included
  • IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

    IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF4G1 antibody included

EIF4G1 Monoclonal antibody, PBS Only

EIF4G1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67199-1-PBS

产品说明书

CloneNo. 2B10G8

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DKFZp686A1451, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EIF4G1 Ready-To-Use IHC Kit. EIF4G1 primary antibody included.

产品信息

67199-1-PBS targets EIF4G1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 EIF4G1 fusion protein Ag8357 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4 gamma, 1
别名 DKFZp686A1451, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220
计算分子量 1600 aa, 176 kDa
观测分子量 240-250 kDa
GenBank蛋白编号BC007788
基因名称 EIF4G1
Gene ID (NCBI) 1981
RRIDAB_2882492
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ04637
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )