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  • KD/KO Validated

EIF4G1 Polyclonal antibody

EIF4G1 Polyclonal Antibody for WB, IP, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF/ICC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15704-1-AP

Print datasheet

Synonyms

eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220



经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells, Ethacrynic acid treated HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF4G1 Ready-To-Use IHC Kit. EIF4G1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15704-1-AP targets EIF4G1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IF/ICC, IHC, ELISA
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen EIF4G1 fusion protein Ag8342 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4 gamma, 1
Synonyms eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220
Calculated Molecular Weight 1600 aa, 176 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberBC007788
Gene Symbol EIF4G1
Gene ID (NCBI) 1981
RRIDAB_2261979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )

实验方案

Product Specific Protocols
WB protocol for EIF4G1 antibody 15704-1-APDownload protocol
IHC protocol for EIF4G1 antibody 15704-1-APDownload protocol
IF protocol for EIF4G1 antibody 15704-1-APDownload protocol
IP protocol for EIF4G1 antibody 15704-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
CoIP

Mol Cell

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

Authors - Linyu Sun
humanIF

J Extracell Vesicles

Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation.

Authors - Bo You
humanWB

Proc Natl Acad Sci U S A

Cellular cap-binding protein, eIF4E, promotes picornavirus genome restructuring and translation.

Authors - Brian C Avanzino
humanWB

PLoS Genet

SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation.

Authors - Siqi Hu
mouseWB,IF

Diabetes

Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.

Authors - Seokwon Jo
  • KO Validated
humanIF

J Cell Sci

ADAR1 limits stress granule formation through both translation-dependent and translation-independent mechanisms.

Authors - Giulia A Corbet