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验证数据展示

  • WB analysis using 67740-1-Ig (same clone as 67740-1-PBS)

    WB analysis using 67740-1-Ig (same clone as 67740-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67740-1-Ig (EIF4A3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67740-1-Ig (same clone as 67740-1-PBS)

    IHC staining of human colon cancer using 67740-1-Ig (same clone as 67740-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67740-1-Ig (EIF4A3 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF4A3 antibody included
  • WB analysis using 67740-1-Ig (same clone as 67740-1-PBS)

    WB analysis using 67740-1-Ig (same clone as 67740-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67740-1-Ig (EIF4A3 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67740-1-Ig (same clone as 67740-1-PBS)

    IF Staining of HepG2 using 67740-1-Ig (same clone as 67740-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EIF4A3 antibody (67740-1-Ig, Clone: 1G6H9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

EIF4A3 Monoclonal antibody, PBS Only

EIF4A3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67740-1-PBS

产品说明书

CloneNo. 1G6H9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EIF4A3 Ready-To-Use IHC Kit. EIF4A3 primary antibody included.

产品信息

67740-1-PBS targets EIF4A3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag11130

Product name: Recombinant human EIF4A3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-411 aa of BC003662

Sequence: MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4A, isoform 3
别名 DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34
计算分子量 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC003662
基因名称 EIF4A3
Gene ID (NCBI) 9775
RRIDAB_2918509
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP38919
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions

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