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EIF4A3 Monoclonal antibody

EIF4A3 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G6H9

Cat No : 67740-1-Ig

Print datasheet

Synonyms

DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34

验证数据展示

  • WB analysis using 67740-1-Ig

    WB analysis using 67740-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67740-1-Ig (EIF4A3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human colon cancer using 67740-1-Ig

    IHC staining of human colon cancer using 67740-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67740-1-Ig (EIF4A3 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF4A3 antibody included
  • WB analysis using 67740-1-Ig

    WB analysis using 67740-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67740-1-Ig (EIF4A3 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67740-1-Ig

    IF Staining of HepG2 using 67740-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EIF4A3 antibody (67740-1-Ig, Clone: 1G6H9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman colon cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF4A3 Ready-To-Use IHC Kit. EIF4A3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:1250-1:5000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67740-1-Ig targets EIF4A3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen EIF4A3 fusion protein Ag11130 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4A, isoform 3
Synonyms DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC003662
Gene Symbol EIF4A3
Gene ID (NCBI) 9775
RRIDAB_2918509
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP38919
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions

实验方案

Product Specific Protocols
WB protocol for EIF4A3 antibody 67740-1-IgDownload protocol
IHC protocol for EIF4A3 antibody 67740-1-IgDownload protocol
IF protocol for EIF4A3 antibody 67740-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

RNA

The kinesin motor KIF1C is a putative transporter of the exon junction complex in neuronal cells

Authors - Maike Nagel
View Article