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验证数据展示

  • WB analysis of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    WB analysis of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10439-1-AP (same clone as 10439-1-PBS)

    IHC staining of human breast cancer using 10439-1-AP (same clone as 10439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10439-1-AP (EIF3J antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    IP experiment of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    IP result of anti-EIF3J (IP:10439-1-AP, 4ug; Detection:10439-1-AP 1:500) with HeLa cells lysate 880ug. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    IF Staining of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF3J antibody (10439-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EIF3J Polyclonal antibody, PBS Only

EIF3J Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10439-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Eukaryotic translation initiation factor 3 subunit 1, EIF3S1, eIF-3-alpha, eIF3 p35, eIF3 alpha

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10439-1-PBS targets EIF3J in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0678

Product name: Recombinant human EIF3J protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-258 aa of BC002719

Sequence: MAAAAAAAGDSDSWDADAFSVEDPVRKVGGGGTAGGDRWEGEDEDEDVKDNWDDDDDEKKEEAEVKPEVKISEKKKIAEKIKEKERQQKKRQEEIKKRLEEPEEPKVLTPEEQLADKLRLKKLQEESDLELAKETFGVNNAVYGIDAMNPSSRDDFTEFGKLLKDKITQYEKSLYYASFLEVLVRDVCISLEIDDLKKITNSLTVLCSEKQKQEKQSKAKKKKKGVVPGGGLKATMKDDLADYGGYDGGYVQDYEDFM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit J
别名 Eukaryotic translation initiation factor 3 subunit 1, EIF3S1, eIF-3-alpha, eIF3 p35, eIF3 alpha
计算分子量 28 kDa
观测分子量 35 kDa
GenBank蛋白编号BC002719
基因名称 EIF3J
Gene ID (NCBI) 8669
RRIDAB_2097082
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75822
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF3 has a key role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex(PMID:17588516). The eIF3 complex stimulates several steps in the translation initiation pathway, including dissociation of 80S ribosomes into 40S and 60S subunits, binding of a ternary complex (TC) consisting of Met-tRNA , eIF2, and GTP to the small subunit (forming the 43S preinitiation complex) and recruitment of mRNA to the 43 S complex to produce the 48S complex (PMID:11560931) EIF3J was identified as a high copy suppressor of the temperature-sensitive (Ts-) phenotype of the rpg1-1 allele of TIF32/RPG1, encoding the largest subunit of yeast eIF3 (eIF3a) (PMID:10488093).

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