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验证数据展示

  • IF Staining of U2OS using CL488-10899

    IF Staining of U2OS using CL488-10899

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 EIF3E antibody (CL488-10899) at dilution of 1:200.

  • IF Staining of SH-SY5Y using CL488-10899

    IF Staining of SH-SY5Y using CL488-10899

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 EIF3E antibody (CL488-10899) at dilution of 1:200.

CoraLite® Plus 488-conjugated EIF3E Polyclonal antibody

EIF3E Polyclonal Antibody for IF/ICC
Cat No. CL488-10899

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells, SH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy EIF3E Ready-To-Use IHC Kit. EIF3E primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10899-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10899 targets EIF3E in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1132

Product name: Recombinant human EIF3E protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 145-445 aa of BC008419

Sequence: SGAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit E
别名 Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48
计算分子量 445 aa, 52 kDa
观测分子量 48-52 kDa
GenBank蛋白编号BC008419
基因名称 EIF3E
Gene ID (NCBI) 3646
RRIDAB_3672470
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60228
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EIF3E antibody CL488-10899Download protocol
Standard Protocols
Click here to view our Standard Protocols