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验证数据展示

  • IP experiment of HeLa using 67095-1-Ig (same clone as 67095-1-PBS)

    IP experiment of HeLa using 67095-1-Ig (same clone as 67095-1-PBS)

    IP result of anti-EIF3E (IP:67095-1-Ig, 5ug; Detection:67095-1-Ig 1:2000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

  • WB analysis using 67095-1-Ig (same clone as 67095-1-PBS)

    WB analysis using 67095-1-Ig (same clone as 67095-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67095-1-Ig (EIF3E antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67095-1-Ig (same clone as 67095-1-PBS)

    IF Staining of HepG2 using 67095-1-Ig (same clone as 67095-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF3E antibody (67095-1-Ig, Clone: 1C6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67095-1-PBS in a different storage buffer formulation.

EIF3E Monoclonal antibody, PBS Only

EIF3E Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 67095-1-PBS

产品说明书

CloneNo. 1C6C8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

1C6C8, eIF-3 p48, EIF3S6, INT6, Viral integration site protein INT-6 homolog

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EIF3E Ready-To-Use IHC Kit. EIF3E primary antibody included.

产品信息

67095-1-PBS targets EIF3E in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28523

Product name: Recombinant human EIF3E protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 146-445 aa of BC000734

Sequence: GAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit E
别名 1C6C8, eIF-3 p48, EIF3S6, INT6, Viral integration site protein INT-6 homolog
计算分子量 48 kDa
观测分子量 48-52 kDa
GenBank蛋白编号BC000734
基因名称 EIF3E
Gene ID (NCBI) 3646
RRIDAB_2882400
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP60228
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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