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验证数据展示

  • IHC staining of human breast cancer using 11352-1-AP

    IHC staining of human breast cancer using 11352-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF3E antibody included
  • WB analysis of HeLa using 11352-1-AP

    WB analysis of HeLa using 11352-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 11352-1-AP

    IP experiment of HepG2 using 11352-1-AP

    IP result of anti-EIF3E (IP:11352-1-AP, 4ug; Detection:11352-1-AP 1:500) with HepG2 cells lysate 880 ug.

  • IF Staining of HepG2 using 11352-1-AP

    IF Staining of HepG2 using 11352-1-AP

    Immunofluorescent analysis of HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

EIF3E Polyclonal antibody

EIF3E Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11352-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF3E Ready-To-Use IHC Kit. EIF3E primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11352-1-AP targets EIF3E in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1856

Product name: Recombinant human EIF3E protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 146-445 aa of BC000734

Sequence: GAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit E
别名 Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48
计算分子量 48 kDa
观测分子量 48-52 kDa
GenBank蛋白编号BC000734
基因名称 EIF3E
Gene ID (NCBI) 3646
RRIDAB_2277716
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60228
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for EIF3E antibody 11352-1-APDownload protocol
IHC protocol for EIF3E antibody 11352-1-APDownload protocol
IP protocol for EIF3E antibody 11352-1-APDownload protocol
WB protocol for EIF3E antibody 11352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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