- Featured Product
- KD/KO Validated
EIF2B4 Polyclonal antibody
EIF2B4 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, K-562 cells, HeLa cells, HL-60 cells, mouse skeletal muscle tissue |
| Positive IHC detected in | human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
产品信息
11332-1-AP targets EIF2B4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IF/ICC, IHC, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | EIF2B4 fusion protein Ag1875 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa |
| Synonyms | EIF 2B, EIF2B, EIF2B4, EIF2BD, EIF2Bdelta, eIF2Bδ |
| Calculated Molecular Weight | 523 aa, 58 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC001870 |
| Gene Symbol | EIF2B4 |
| Gene ID (NCBI) | 8890 |
| RRID | AB_2277640 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UI10 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| IHC protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| IF protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell The HRI branch of the integrated stress response selectively triggers mitophagy
| ||
Elife Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response. | ||
Elife The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes. | ||
ACS Cent Sci Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b. | ||
