验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, K-562 cells, HeLa cells, HL-60 cells, mouse skeletal muscle tissue |
| Positive IHC detected in | human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
产品信息
11332-1-AP targets EIF2B4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1875 Product name: Recombinant human EIF2B4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-341 aa of BC001870 Sequence: MAAVAVAVREDSGSGMKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPETGSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKGEQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa |
| 别名 | Translation initiation factor eIF2B subunit delta, EIF2Bdelta, EIF2BD, eIF2B GDP-GTP exchange factor subunit delta, EIF2B |
| 计算分子量 | 523 aa, 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC001870 |
| 基因名称 | EIF2B4 |
| Gene ID (NCBI) | 8890 |
| RRID | AB_2277640 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UI10 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| IHC protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| WB protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell The HRI branch of the integrated stress response selectively triggers mitophagy
| ||
Elife Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response. | ||
Elife The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes. | ||
ACS Cent Sci Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b. | ||
