• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 83806-1-RR (same clone as 83806-1-PBS)

    WB analysis using 83806-1-RR (same clone as 83806-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83806-1-RR (EGFR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

    IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer.​ This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83806-1-RR (same clone as 83806-1-PBS)

    IF Staining of A431 using 83806-1-RR (same clone as 83806-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR-Specific antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

    IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

    IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EGFR antibody included
  • FC experiment of A431 using 83806-1-RR (same clone as 83806-1-PBS)

    FC experiment of A431 using 83806-1-RR (same clone as 83806-1-PBS)

    1x10^6 A431 cells (left) or Jurkat cells (right) were intracellularly stained with 0.4 ug EGFR Recombinant antibody (83806-1-RR, Clone: 240603F10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

    IHC staining of human placenta using 83806-1-RR (same clone as 83806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83806-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EGFR antibody included

EGFR Recombinant monoclonal antibody, PBS Only

EGFR Uni-rAb® Recombinant Antibody for WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA
Cat No. 83806-1-PBS

产品说明书

CloneNo. 240603F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

别名

EGFR-Specific, 240603F10, EGF R, EGF Receptor , Epidermal growth factor receptor

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

产品信息

83806-1-PBS targets EGFR in WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 EGFR-Specific, 240603F10, EGF R, EGF Receptor , Epidermal growth factor receptor
计算分子量 134 kDa
观测分子量145-165 kDa
GenBank蛋白编号NM_005228
基因名称 EGFR
Gene ID (NCBI) 1956
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP00533
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.

||
New chat Able
正在加载，请稍候...