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验证数据展示

  • WB analysis using 83806-1-RR

    WB analysis using 83806-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83806-1-RR (EGFR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 83806-1-RR

    IHC staining of human placenta using 83806-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer.​

  • IF Staining of A431 using 83806-1-RR

    IF Staining of A431 using 83806-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR-Specific antibody (83806-1-RR, Clone: 240603F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human placenta using 83806-1-RR

    IHC staining of human placenta using 83806-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer.

  • IHC staining of human placenta using 83806-1-RR

    IHC staining of human placenta using 83806-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EGFR antibody included
  • FC experiment of A431 using 83806-1-RR

    FC experiment of A431 using 83806-1-RR

    1x10^6 A431 cells (left) or Jurkat cells (right) were intracellularly stained with 0.4 ug EGFR Recombinant antibody (83806-1-RR, Clone: 240603F10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA.

  • IHC staining of human placenta using 83806-1-RR

    IHC staining of human placenta using 83806-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83806-1-RR (EGFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EGFR antibody included

EGFR Recombinant monoclonal antibody

EGFR Uni-rAb® Recombinant Antibody for WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA
Cat No. 83806-1-RR

产品说明书

CloneNo. 240603F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

别名

EGFR-Specific, 240603F10, EGF R, EGF Receptor , Epidermal growth factor receptor

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IHC-Autostainer detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HaCaT cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)-AUTOSTAINERIHC-AUTOSTAINER : 1:50-1:500
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83806-1-RR targets EGFR in WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 EGFR-Specific, 240603F10, EGF R, EGF Receptor , Epidermal growth factor receptor
计算分子量 134 kDa
观测分子量145-165 kDa
GenBank蛋白编号NM_005228
基因名称 EGFR
Gene ID (NCBI) 1956
RRIDAB_3671393
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP00533
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.

实验方案

Product Specific Protocols
IF protocol for EGFR antibody 83806-1-RRDownload protocol
IHC protocol for EGFR antibody 83806-1-RRDownload protocol
WB protocol for EGFR antibody 83806-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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