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Pikachurin Monoclonal antibody

Pikachurin Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C6D12

Cat No : 66232-1-Ig

Print datasheet

Synonyms

Agrin like protein, AGRINL, AGRNL, EGFLAM, Pikachurin

验证数据展示

  • IF Staining of Y79 using 66232-1-Ig

    IF Staining of Y79 using 66232-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of human retina using 66232-1-Ig

    WB analysis of human retina using 66232-1-Ig

    human retina tissue were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman retina tissue, PC-3 cells
Positive IF detected inY79 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66232-1-Ig targets Pikachurin in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen Pikachurin fusion protein Ag6362 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name EGF-like, fibronectin type III and laminin G domains
Synonyms Agrin like protein, AGRINL, AGRNL, EGFLAM, Pikachurin
Calculated Molecular Weight 111 kDa
Observed Molecular Weight 111 kDa, 55-60 kDa
GenBank Accession NumberBC033177
Gene Symbol EGFLAM
Gene ID (NCBI) 133584
RRIDAB_2881623
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ63HQ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).

实验方案

Product Specific Protocols
WB protocol for Pikachurin antibody 66232-1-IgDownload protocol
IF protocol for Pikachurin antibody 66232-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols