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验证数据展示

  • WB analysis of human brain using 12480-1-AP

    WB analysis of human brain using 12480-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis using 12480-1-AP

    WB analysis using 12480-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 12480-1-AP

    IHC staining of human skeletal muscle using 12480-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12480-1-AP (Ephrin A3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 12480-1-AP

    IHC staining of human skeletal muscle using 12480-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12480-1-AP (Ephrin A3 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of A549 using 12480-1-AP

    IP experiment of A549 using 12480-1-AP

    IP result of anti-Ephrin A3 (IP:12480-1-AP, 4ug; Detection:12480-1-AP 1:500) with A549 cells lysate 1040ug.

  • IF Staining of mouse spleen using 12480-1-AP

    IF Staining of mouse spleen using 12480-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Ephrin A3 antibody (12480-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 12480-1-AP

    IF Staining of mouse spleen using 12480-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Ephrin A3 antibody (12480-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

Ephrin A3 Polyclonal antibody

Ephrin A3 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 12480-1-AP

产品说明书

引用文献(7)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA and More (1)

别名

EFL2, EFNA3, EPLG3, LERK3, EFL 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12480-1-AP targets Ephrin A3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原

CatNo: Ag3188

Product name: Recombinant human EFNA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-222 aa of BC017722

Sequence: LGNRHAVYWNSSNQHLRREGYTVQVNVNDYLDIYCPHYNSSGVGPGAGPGPGGGAEQYVLYMVSRNGYRTCNASQGFKRWECNRPHAPHSPIKFSEKFQRYSAFSLGYEFHAGHEYYYISTPTHNLHWKCLRMKVFVCCASTSHSGEKPVPTLPQFTMGPNVKINVLEDFEGENPQVPKLEKSISGTSPKREHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ephrin-A3
别名 EFL2, EFNA3, EPLG3, LERK3, EFL 2
计算分子量 238 aa, 26 kDa
观测分子量 26-35 kDa
GenBank蛋白编号BC017722
基因名称 Ephrin A3
Gene ID (NCBI) 1944
RRIDAB_10638305
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52797
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Ephrin A3 antibody 12480-1-APDownload protocol
IHC protocol for Ephrin A3 antibody 12480-1-APDownload protocol
IP protocol for Ephrin A3 antibody 12480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Research (Wash D C)

Exercise-Induced miR-210 Promotes Cardiomyocyte Proliferation and Survival and Mediates Exercise-Induced Cardiac Protection against Ischemia/Reperfusion Injury

Authors - Yihua Bei
View Article
humanWB

Cancer Med

EphrinA3 is a key regulator of malignant behaviors and a potential prognostic factor in lung adenocarcinoma.

Authors - Ruzetuoheti Yiminniyaze
View Article

JCI Insight

Erythromycin inhibits neutrophilic inflammation and mucosal disease by upregulating DEL-1.

Authors - Tomoki Maekawa
View Article
humanWB

Biomed Res Int

OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway.

Authors - Hui Wang
  • KD Validated
View Article
humanWB,IF

J Exp Clin Cancer Res

The Ephrin tyrosine kinase a3 (EphA3) is a novel mediator of RAGE-prompted motility of breast cancer cells

Authors - Marianna Talia
  • KD Validated
View Article
ratWB

Brain Res Bull

Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats

Authors - Tao Tong
View Article