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验证数据展示

  • WB analysis of HepG2 using 24296-1-AP

    WB analysis of HepG2 using 24296-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24296-1-AP (EFCAB5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 24296-1-AP

    IHC staining of human liver cancer using 24296-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50.

  • IHC staining of human liver cancer using 24296-1-AP

    IHC staining of human liver cancer using 24296-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50.

  • IF Staining of HepG2 using 24296-1-AP

    IF Staining of HepG2 using 24296-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EFCAB5 antibody (24296-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

EFCAB5 Polyclonal antibody

EFCAB5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

EF-hand calcium-binding domain-containing protein 5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman liver cancer tissue, human colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24296-1-AP targets EFCAB5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19274

Product name: Recombinant human EFCAB5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-342 aa of BC137519

Sequence: MNESASQEELRPAQENRKEDKERKWNLTEVKELHETLQSVPDVPVKEDTNSVVEKAMDEIKSQELNLEGQRKISPGSIKDSKTEASGNIAIRKSAKVIFALDETELKSKPEHTWKKNLFERMEARAQAMQQKIIDKENLKKELEKKAEKKLPRDNLAKEWFNTDSMTLNNTAYLLDKLLPTLVPGVENMLTQVEKKKVLTEADTPSKFDPINYLGEYLIRNNPNYIKDPGMSGYQRLMKEVTEDLKIYVPDTICNRVSKMKENVKQNRKQRESIDKIKVKVANTRKQALQEQFDEWILDPKGMIPKSVIQNVLQEFFQNPDFKLGSHCKQLDITDSTEPRLN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EF-hand calcium binding domain 5
别名 EF-hand calcium-binding domain-containing protein 5
计算分子量 1503 aa, 173 kDa
观测分子量 110 kDa
GenBank蛋白编号BC137519
基因名称 EFCAB5
Gene ID (NCBI) 374786
RRIDAB_2879479
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDA4FU69
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for EFCAB5 antibody 24296-1-APDownload protocol
IHC protocol for EFCAB5 antibody 24296-1-APDownload protocol
WB protocol for EFCAB5 antibody 24296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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