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验证数据展示

  • IF Staining of HeLa using CL488-68148

    IF Staining of HeLa using CL488-68148

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 EEF1G antibody (CL488-68148, Clone: 1G7C6 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated EEF1G Monoclonal antibody

EEF1G Monoclonal Antibody for IF/ICC
Cat No. CL488-68148

产品说明书

CloneNo. 1G7C6

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Pig, Rabbit

应用

IF/ICC

别名

eEF 1B gamma, EEF1G, EF 1 gamma, EF1G, Elongation factor 1 gamma, GIG35

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68148-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68148 targets EEF1G in IF/ICC applications and shows reactivity with Human, Pig, Rabbit samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Pig, Rabbit
免疫原

CatNo: Ag0218

Product name: Recombinant human EEF1G protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 140-400 aa of BC006520

Sequence: GLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWR

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 1 gamma
别名 eEF 1B gamma, EEF1G, EF 1 gamma, EF1G, Elongation factor 1 gamma, GIG35
计算分子量 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC006520
基因名称 EEF1G
Gene ID (NCBI) 1937
RRIDAB_3084419
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP26641
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EEF1G antibody CL488-68148Download protocol
Standard Protocols
Click here to view our Standard Protocols