CoraLite®594-conjugated EEF1B2 Monoclonal antibody
EEF1B2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3E1C11
验证数据展示
经过测试的应用
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy EEF1B2 Ready-To-Use IHC Kit. EEF1B2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60329-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-60329 targets EEF1B2 in IF applications and shows reactivity with human samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | human |
| Immunogen | EEF1B2 fusion protein Ag0135 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | eukaryotic translation elongation factor 1 beta 2 |
| Synonyms | EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 30-34 kDa |
| GenBank Accession Number | BC000211 |
| Gene Symbol | EEF1B2 |
| Gene ID (NCBI) | 1933 |
| RRID | AB_2883465 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P24534 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 EEF1B2 antibody CL594-60329 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |