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CoraLite® Plus 488-conjugated EEF1A1 Monoclonal antibody

EEF1A1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A9F5

Cat No : CL488-67495

Print datasheet

Synonyms

CCS 3, CCS3, EEF 1, EEF1A, eEF1A 1, EEF1A1, EF 1 alpha 1, EF Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, FLJ25721, GRAF 1EF, HNGC:16303, LENG7, PTI1

验证数据展示

  • IF Staining of HepG2 using CL488-67495

    IF Staining of HepG2 using CL488-67495

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 EEF1A1 antibody (CL488-67495, Clone: 1A9F5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67495

    FC experiment of HeLa using CL488-67495

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human EEF1A1 (CL488-67495, Clone:1A9F5) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using CL488-67495

    FC experiment of HepG2 using CL488-67495

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human EEF1A1 (CL488-67495, Clone:1A9F5) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EEF1A1 Ready-To-Use IHC Kit. EEF1A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67495-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67495 targets EEF1A1 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen EEF1A1 fusion protein Ag1938 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation elongation factor 1 alpha 1
Synonyms CCS 3, CCS3, EEF 1, EEF1A, eEF1A 1, EEF1A1, EF 1 alpha 1, EF Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, FLJ25721, GRAF 1EF, HNGC:16303, LENG7, PTI1
Calculated Molecular Weight 462 aa, 50 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC014224
Gene Symbol EEF1A1
Gene ID (NCBI) 1915
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP68104
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EEF1A1 antibody CL488-67495Download protocol
FC protocol for CL Plus 488 EEF1A1 antibody CL488-67495Download protocol
Standard Protocols
Click here to view our Standard Protocols