• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 81245-1-RR (same clone as 81245-1-PBS)

    WB analysis using 81245-1-RR (same clone as 81245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81245-1-RR (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81245-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81245-1-RR (same clone as 81245-1-PBS)

    WB analysis of HepG2 using 81245-1-RR (same clone as 81245-1-PBS)

    WB result of EEA1 antibody (81245-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells. This data was developed using the same antibody clone with 81245-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81245-1-RR

    Affinity and Kinetic Characterization of 81245-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81245-1-RR against Human EEA1 were performed. The affinity constant is 0.227 nM.

  • Featured Product
  • KD/KO验证
    • {{ptg:ProductFeaturesInfo}}

EEA1 Recombinant monoclonal antibody, PBS Only

EEA1 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 81245-1-PBS

产品说明书

CloneNo. 2C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

81245-1-PBS targets EEA1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28814

Product name: Recombinant human EEA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1151-1350 aa of NM_003566

Sequence: KLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 early endosome antigen 1
别名 ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1
计算分子量 162 kDa
观测分子量 162 kDa
GenBank蛋白编号NM_003566
基因名称 EEA1
Gene ID (NCBI) 8411
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15075
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.