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EDEM2 Polyclonal antibody

EDEM2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11241-1-AP

Print datasheet

Synonyms

C20orf31, C20orf49, EDEM2

验证数据展示

  • WB analysis using 11241-1-AP

    WB analysis using 11241-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11241-1-AP (EDEM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 11241-1-AP

    IHC staining of human heart using 11241-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11241-1-AP (EDEM2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 11241-1-AP

    IHC staining of human heart using 11241-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11241-1-AP (EDEM2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells, mouse skeletal muscle tissue, U-251 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11241-1-AP targets EDEM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen EDEM2 fusion protein Ag1755 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ER degradation enhancer, mannosidase alpha-like 2
Synonyms C20orf31, C20orf49, EDEM2
Calculated Molecular Weight64.8 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC016184
Gene Symbol EDEM2
Gene ID (NCBI) 55741
RRIDAB_2877752
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EDEM2 antibody 11241-1-APDownload protocol
IHC protocol for EDEM2 antibody 11241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols