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验证数据展示

  • WB analysis using 11241-1-AP

    WB analysis using 11241-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11241-1-AP (EDEM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 11241-1-AP

    IHC staining of human heart using 11241-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11241-1-AP (EDEM2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 11241-1-AP

    IHC staining of human heart using 11241-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11241-1-AP (EDEM2 Antibody) at dilution of 1:50 (under 40x lens).

EDEM2 Polyclonal antibody

EDEM2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11241-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C20orf31, C20orf49, EDEM2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, mouse skeletal muscle tissue, U-251 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11241-1-AP targets EDEM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EDEM2 fusion protein Ag1755 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ER degradation enhancer, mannosidase alpha-like 2
别名 C20orf31, C20orf49, EDEM2
计算分子量64.8 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC016184
基因名称 EDEM2
Gene ID (NCBI) 55741
RRIDAB_2877752
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BV94
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EDEM2 antibody 11241-1-APDownload protocol
IHC protocol for EDEM2 antibody 11241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols