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EDEM1 Polyclonal antibody

EDEM1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 26226-1-AP

Print datasheet

Synonyms

EDEM, EDEM1, KIAA0212

验证数据展示

  • WB analysis using 26226-1-AP

    WB analysis using 26226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26226-1-AP (EDEM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 26226-1-AP

    IHC staining of human liver cancer using 26226-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26226-1-AP (EDEM1 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse liver using 26226-1-AP

    IP experiment of mouse liver using 26226-1-AP

    IP result of anti-EDEM1 (IP:26226-1-AP, 4ug; Detection:26226-1-AP 1:500) with mouse liver tissue lysate 4000ug.

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, mouse liver, rat liver
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

The immunogen of 26226-1-AP is EDEM1 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen EDEM1 fusion protein Ag24046 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ER degradation enhancer, mannosidase alpha-like 1
Synonyms EDEM, EDEM1, KIAA0212
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 66-75 kDa
GenBank Accession NumberBC019088
Gene Symbol EDEM1
Gene ID (NCBI) 9695
RRIDAB_2880433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92611
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EDEM1 antibody 26226-1-APDownload protocol
IHC protocol for EDEM1 antibody 26226-1-APDownload protocol
IP protocol for EDEM1 antibody 26226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogene

Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene.

Authors - Chengdong Liu
View Article
humanWB

Front Mol Biosci

Glutamate-Cysteine Ligase Catalytic Subunit Attenuated Hepatitis C Virus-Related Liver Fibrosis and Suppressed Endoplasmic Reticulum Stress.

Authors - Na Fu
View Article
humanWB

J Cell Biochem

Erdj3 has an essential role for Z variant Alpha-1-Antitrypsin Degradation.

Authors - Nazli Khodayari
View Article
mouseWB

bioRxiv

A new role for IFRD1 in regulation of ER stress in bladder epithelial homeostasis

Authors - Bisiayo E Fashemi
View Article